Establishment of method for determining serum beta-hydroxybutyric acid and its meaning in diabetes ketosis acidism
- VernacularTitle:血清β-羟丁酸测定方法的建立及其在糖尿病酮症酸中毒中的意义
- Author:
Bin YI
;
Guifen WEI
;
Yin TANG
- Publication Type:Journal Article
- From:
Journal of Central South University(Medical Sciences)
2001;26(2):185-186
- CountryChina
- Language:Chinese
-
Abstract:
Objective This study was to establish enzyme-rate method for determining serum beta-hydroxybutyric acid ,and to discuss its meaning in diabetes ketosis acidism. Methods Enzyme-rate method was used to determine serum beta-hydroxybutyric acid in 60 cases of normals, 30 cases of diabetes ,and 8 cases of ketosis acidism by Hitachi 7170A autochemistry analyzer. Results The recovery rate is 108%,the linerity extent 0-4.0 mmol.L-1, the coefficient of variation within-run is 2.2%and day-to-day is 2.6%. Conclusion The enzyme-rate method for determining serum beta-hydroxybutyric acid save time, gives accurate results and has wide linerity,so it can used for diagnosis of diabetes ketosis acidism.
