INDUCTION OF APOPTOSIS OF HUMAN LIVER CARCINOMA CELLS BY TEA POLYPHENOLS

VernacularTitle:茶多酚诱导肝癌细胞凋亡
Author: Qin WU ; Tiquan FU ; Chuanqian FENG
Publication Type:Journal Article
From: Medical Journal of Chinese People's Liberation Army 2001;26(2):110-112
CountryChina
Language:Chinese
Abstract: Tea polyphenols were tried to induce apoptosis of human cultured hepatocellular carcinoma cell line HepG-Ⅱ. MTT assay, DNA agarose gel electrophoresis, transmission electron microscopy , fluorescence decoration and DNA end labeling method (Tunel) were used to identify apoptosis. Having been treated by tea polyphenols in 250μg/ml , HepG-Ⅱcell apoptosis was induced. The induction of apoptosis was the dose dependent. Chromatin condensation, apoptotic body formation, fluorescence of yellow green and pellet were observed. Agarose gel electrophoresis analysis revealed DNA cleavages( DNA ladder). The results indicated that tea polyphenole could induce apoptosis of cultured human hepatocellular carcinoma cell line HepG-Ⅱ. It suggested that tea polyphenols could be used to treat human liver carcinoma.