Macrophage colony-stimulating factor protects RAW264.7 cells from oxidative injury through the induction of MnSOD expression
- VernacularTitle:M-CSF诱导MnSOD表达减轻RAW264.7细胞氧化损伤
- Author:
Zhanjun PANG
;
Fuqi XING
- Publication Type:Journal Article
- From:
Basic & Clinical Medicine
2001;21(2):172-175
- CountryChina
- Language:Chinese
-
Abstract:
To show the protective effect of macrophage colony-stimulating factor (M-CSF) on macrophages under oxidative stress,we investigated the effect of M-CSF on RAW264.7 cells incubated with tert-butyl hydroperoxide (tbOOH) using L929 cell conditioned medium (L929-CM) as the source of M-CSF.The results showed that M-CSF could alleviate the tbOOH- induced oxidative injury to RAW264.7 cells.We also found that M-CSF could improve superoxide dismutase (SOD) activity in the cells.MnSOD mRNA expression was also shown to be increased by RT-PCR technique,and the induction could be blocked by actinomycin D.So,we concluded that M-CSF could protect RAW264.7 cells from oxidative injury through inducing MnSOD expression.
