PRELIMINARY STUDY TO THE PROGRAMMED CELL DEATH OF HL-60 CELL AND BGS-180 CELL INDUCED BY VP-16

VernacularTitle:足叶已甙致人胃低分化腺癌细胞和慢性淋巴细胞性白血病细胞凋亡对比观察
Author: Zhongsheng LU ; Yingcai HUANG
Publication Type:Journal Article
From: Medical Journal of Chinese People's Liberation Army 2001;26(2):117-118
CountryChina
Language:Chinese
Abstract: Programmed cell death(PCD)of human leukemic HL-60 cell and human poorly differentiated gastric adenocarcinoma cell BGS-180 induced by efoposid(VP-16) was preliminarily observed comparatively in the same experimental condition through rate of cell death, DNA agarose gels electrophoresis and terminal deoxynucleotidyl transferase(TdT) mediated biotin-11-dUTP nickend labeling(TUNEL).It was found that apoptosis is the main pattern of HL-60 cell death induced by low does VP-16 in a short time, and it can be suppressed when protein kinase C(PKC) is activated. The main pattern of BGS-180 cell death induced by VP-16 is necrosis, and PCK activation does not affect its necrosis rate. Extracellular Ca2+ reduction do not affect BGS-180 and HL-60 cell death rate. The mechanism of VP-16 action on BGS-180 and HL-60 cell is different, apoptosis is not the main pattern of cell BGS-180 death induced by VP-16.