bcl-xs mediated sensitization of human nasopharyngeal carcinoma cells to camptothecin-induced apoptosis
- VernacularTitle:bcl-xs增加鼻咽癌细胞对喜树碱诱导凋亡敏感性的实验研究
- Author:
Yuefei ZHANG
;
Yangchao CHEN
;
Keyuan ZHOU
;
Tong LIANG
- Publication Type:Journal Article
- From:
Chinese Archives of Otolaryngology-Head and Neck Surgery
2001;8(2):114-117
- CountryChina
- Language:Chinese
-
Abstract:
Objective: In order to detect the effect of bcl-xs on camptothecin-induced apoptosis in human nasopharyngeal carcinoma CNE-2Z cells in vitro.Methods:bcl-xs gene-bearing mammalian expression vector(pcDNA3xs)was transfected into CNE-2Z cells using LipofectAmine.The expression of bcl-xs was determined with western blot.Cells which were transfected with native pcDNA3 vector were used as control.Apoptotic cells were detected with flow cytometry after exposure to camptothecin for 24h.Results:Cell clone(CNE-2Zxs)with stable expression of bcl-xs was obtained as confirmed with western blot.Results from flow cytometry analysis showed a significant increase of apoptotic cells in CNE-2Zxs as compared with CNE-2Zneo after treatment with the same dose of camptothecin.Conclusion:Exogenous bcl-xs expression sensitized nasopharyngeal carcinoma CNE-2Z cells to camptothecin-induced apoptosis.
