The Interaction of 5-Fluorouracil with Deoxyribonucleic Acid and Serum Albumin
- VernacularTitle:金属离子存在下5-氟尿嘧啶与脱氧核糖核酸、血清白蛋白的相互作用
- Author:
Jixin LU
;
Guizhu ZHANG
;
Yuemei WANG
- Publication Type:Journal Article
- From:
Chinese Journal of Analytical Chemistry
2001;29(2):192-194
- CountryChina
- Language:Chinese
-
Abstract:
The interaction of 5-fluorouracil (5-FU) with deoxyribonucleic acid (DNA) and serum albumin and the influence of some metal ions on the interaction have been studied by using spectral methods of UV-absorption,fluorescence and circular dichroism. It was shown that the interaction of 5-FU with DNA and serum albumin was effected by some metal ions. The binding costant of 5-FU with serum albumin was found to be KHSA = 4.38 × 103(mol/L)-1 and KBSA =4.95 × 103(mol/L)-1 and the distance between the bound 5-FU and the tryptophan residue was also determined to be rHSA = 3.53 nm, rBSA = 3.51 nm.
