Determination of Monoamine Neurotransmitters and Related Compounds in Microdialysis Solution by High Performace Liquid Chromatography with Pulsed Amperometric Detection
- VernacularTitle:高效液相色谱-脉冲安培检测法测定脑微透析溶液中单胺类神经递质
- Author:
Guiyun XU
;
Xiyan CHANG
;
Liwen CHANG
- Publication Type:Journal Article
- From:
Chinese Journal of Analytical Chemistry
2001;29(2):199-201
- CountryChina
- Language:Chinese
-
Abstract:
A method for determinattion of monoamine neurotransmitters and related compounds in microdialysis solution by high performance liquid chromatography (HPLC) with pulsed amperometric detection was described. A comparison of response of iso potential anperometric (AD) and pulsed amperometric detection (PAD) was made. The results indicated that the sensitivity of PAD was 1.2 fold that of AD. The quantitative determination of monoamine neurotransmitters and related compounds was carried out with 3,4-dihydroxybenzylamine (DHBA) as internal standerd. The recoveries of monoamine neurotransmitters in microdialysis solution are 71% ～ 101%.
