Angiotensin Ⅱ and its Antagonists on Cultured Cardiac M yocytes Hypertrophy
- VernacularTitle:血管紧张素Ⅱ及其受体拮抗剂对心肌细胞肥大的影响
- Author:
Naili DU
;
Wenhang QI
;
Dingliang ZHU
;
Pingjin GAO
;
Xisheng QIU
- Publication Type:Journal Article
- From:Chinese Journal of Hypertension
2001;9(2):130-133
- CountryChina
- Language:Chinese
-
Abstract:
Aim To investigate the effects of angiotensin Ⅱ(AngⅡ),AT1 receptor antagonist losartan and AT2 receptor antagonist PD123177 on protein synthesis rate and AT1 receptor mRNA expression in cultured neonatal rat cardi ac myocytes. Methods The protein synthesis rate in cultured cardiac myocytes w as determined by the3H-leucine incorporation,AT1 receptor mRNA expressi on of cardiac myocytes was assessed by reverse transcription-polymerase chain reaction(RT-PCR). Results AngⅡ increased the 3H-leucine incorporation in cultured cardiac myocytes in a dose dependent manner,losartan but not PD12317 7 could significantly inhibit AngⅡ induced protein synthesis,;AT1 receptor mRNA expression was upregulated after AngⅡ,and inhibited by losartan,but not PD123177. Conclusion AngⅡ can induce cardiac myocytes hypertrophy via upregulating AT1 receptor,and AT1 receptor antagonist can decrease AT1 rec eptor expression,reduce cardiac myocytes hypertrophy.
