Study on in vitro nitric oxide and interleukin-6 levels Induced from peripheral Mononuclear cells in patients with osteosarcoma

VernacularTitle:骨肉瘤患者外周血单个核细胞IL-6和NO的测定
Author: Tao XIAO ; Jianzhen FANG ; Kanghua LI
Publication Type:Journal Article
From: Journal of Central South University(Medical Sciences) 2001;26(2):139-140
CountryChina
Language:Chinese
Abstract: Peripheral blood mononuclear cells from 32 cases of osteosarcoma and 20 normal controls were separated and induced by lipopolysaccharide, followed by 48 hour incubation in vitro, then the supernatant were collected. The levels of IL-6 were measured by enzyme linked immunosorbent assays, the concentrations of NO were measured by Griess methods. The results were as follows: The concentrations of IL-6 and NO were significantly higher than those in normal contrls (P<0.01). There were positive correlation between the levels of IL-6 and NO in patients with osteosarcoma (r=0.652, P<0.01). The results suggest that the immune function of peripheral mononuclear cell in patients with osteosarcoma was disordered. It may activate peripheral mononuclear cells to produce high levels of IL-6 and NO, which may take part in the pathogenesis of osteosarcoma.