Mechanism of chitosan inhibiting rabbit aortic smooth muscle cell prolif eration
- VernacularTitle:壳多糖抑制兔主动脉平滑肌细胞增殖机制的探讨
- Author:
Daxin WANG
;
Zonggui WU
;
Bin ZHOU
;
Yuanying JIAN
;
Ming YING
;
Minghe CHENG
- Publication Type:Journal Article
- From:
Academic Journal of Second Military Medical University
2001;22(2):159-160
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To study the mechanism of chitosan i n inhibiting the proliferation of rabbit aortic smooth muscle cells(SMCs). Methods: By means of c-myc probe labelled with random primers and Northern blot hybridization, we examined the effect of chitosan on vascu lar SMC c- myc mRNA expression, which was stimulated by newborn bull serum (NB S,20%). Results: The oncogene c-myc mRNA expression incerased in cultured vascular SMC 24 h after NBS exposure. These effects were inhibite d by chitosan (20 μg/ml). Conclusion: Chitosan might inhibit the expression of vascular SMC c-myc mRNA stimulated by NBS, through which the proliferation of vascular SMC are inhibited.
