High performance liquid chromatography analysis of porcine platelet-derived gro wth factor and its effect on DNA synthesis of human vascular endothelial cells
- VernacularTitle:猪血小板衍生生长因子的高效液相色谱分析及对血管内皮细胞DNA合成的影响
- Author:
Yusheng REN
;
Zonggui WU
;
Fang CUI
;
Guoliang JIA
;
Shiqiang YU
;
Qiang CHEN
- Publication Type:Journal Article
- From:
Academic Journal of Second Military Medical University
2001;22(2):155-158
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To determine the molecular weight and p urity of porcine platelet-derived growth factor (pPDGF) and to investigate its effect on DNA synthesis of human umbilical vein endothelial cells. Metho ds: In the present experiment, the high performance liquid chromatograph y was used and the molecular weight and purity of pPDGF were studied. Human umbi lical vein endothelial cells was cultured and effects of pPDGF on DNA synthesis of endothelial cells was observed by 3H-TdR incorporation in vitro. Results: The findings of high performance liquid chromatography showed that the molecular weight of pPDGF was 29 120 and the purity was 89.46%, a nd pPDGF significantly promoted DNA synthesis of quiescent endothelial cells wit h a maximal response at a concentration of 40 ng/ml at 48 h. Conclusion: The molecular weight of pPDGF is 29 120, and it can promote DNA synthes is of cultured human umbilical vein endothelial cells.
