Effects of hyaluronidase and hyaluronan on proliferation of va scular endothelial cells and the expression of CD44
- VernacularTitle:透明质酸酶和透明质酸对血管内皮细胞增殖及CD44表达的影响
- Author:
Yongmei WANG
;
Zonggui WU
;
Li LI
;
Lingzhen ZHANG
;
Renqian ZHONG
- Publication Type:Journal Article
- From:
Academic Journal of Second Military Medical University
2001;22(2):144-147
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the effects of hyaluroni dase (HAase) and hyaluronan (HA) on proliferation of vascular endothelial cells and its mechanism. Methods: The cultured bovine aortic endothel ial cells (BAEC) were treated with HAase or HA. Cell proliferation rate was dete cted by MTT assay. The expression of CD44 and DNA content of the cells were meas ured by flow cytometry (FCM). Results: HAase (50 μg/ml) stimula ted cell proliferation ［(50.10±1.23）% vs control, P<0.01］, incre ased S phase cell rate and induced the expression of CD44, but HA (100 μg/ml) i nhibited cell proliferation and the expression of CD44. Conclusion: HAase may degrade antiangiogenic HA of extracellular matrix, which may stim ulate proliferation of endothelial cells and enhance the curative effect of grow th factors to myocardial ischemia.
