Time-dependent change in expression of type-Ⅰ collagen protein gene during the development of restenosis after arterial injury
- VernacularTitle:动脉损伤后Ⅰ型胶原蛋白基因表达在再狭窄形成中的时相性变化
- Author:
Yawen ZHANG
;
Guoyuan ZHANG
;
Zonggui WU
- Publication Type:Journal Article
- From:
Academic Journal of Second Military Medical University
2001;22(2):133-137
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To observe the alteration of type- Ⅰ collagen protein gene expression after arterial injury and investigate its effect on the development of restenosis. Methods: Firstly, thee xperimental carotid arterial injury rabbit model was constructed. Then, Norther n blot, in situ hybridization and histomorphometric analysis were used to de tect the expression of procollagen mRNA and the accumulation of collagen protein 1,2,4 weeks after injury. Results: Type- Ⅰ collag en mRNA increased 1 week after injury, peaked 2 weeks later and decreased 4 week s later. The deposition of the collagen protein account for a high percentage o f space in neointima on histomorphometric analysis. Conclusion: Collagen protein may play an important role in the development of neointima and restenosis.
