Effects of cytokines on the expression of CD40 and CD40 ligand in cultured human monocytes/macrophages
- VernacularTitle:细胞因子对人单核/巨噬细胞表达CD40和CD40L的影响
- Author:
Jinchuan YAN
;
Zonggui WU
;
Jinming CHEN
;
Chao HUANG
;
Lingzhen ZHANG
;
Li LI
;
Renqian ZHONG
- Publication Type:Journal Article
- From:
Academic Journal of Second Military Medical University
2001;22(2):127-129
- CountryChina
- Language:Chinese
-
Abstract:
Objective： To investigate the effect of c ytokines (IFN-γ，TNF and IL-1) on the expression of CD40 and CD40 ligand (CD4 0L) in monocytes/macrophages. Methods： The mRNA expression of C D40 and CD40L was measured by RT-PCR and the CD40，CD40L expression on the mono cytes/macrophages were detected by flow cytometric analysis. Results： IFN-γ，TNF and IL-1 could not only significantly up-regulate the mRNA levels of CD40 and CD40L in cultured monocytes/macrophages, but also increase t he expression of CD40 and CD40L. Antioxidant VitE could reduce the expression o f CD40 and CD40L induced by IFN-γ，TNF and IL-1. Conclusion： IFN-γ，TNF and IL-1 can stimulate high expression of CD40 and CD40L . Antio xidant VitE can partially inhibit the expression of CD40 and CD40L induced by cy tokines in cultured monocytes/macrophages.
