Insulin stimulates translocation of GLUT4 and glucose uptake in ischemic myocar dium in dog
- VernacularTitle:胰岛素促进犬缺血心肌GLUT4移位和葡萄糖摄取
- Author:
Renfu YIN
;
Jinming CHEN
;
Zonggui WU
;
Shaohua QIU
;
Yuanxin LI
;
Xiaoyue HU
- Publication Type:Journal Article
- From:
Academic Journal of Second Military Medical University
2001;22(2):112-114
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate whether insulin stimulates the translocation of glucose transporter-4 (GLUT4) and glucose uptak e in ischemic myocardium. Methods: Plasma concentration of gluc ose, lactate, free fatty acid and insulin were determined by autoanalyser, and G LUT4 was studied by Western blotting analysis. Results: Insulin increased GLUT4 significantly in sarcolemma of ischemic myocardium ［(25±4)% vs (40±6)%］, and GLUT4 content in intracellular membrane decreased proporti onally. The glucose uptake increased significantly in insulin-ischemic myocardi um. The uptake of insulin-ischemic myocardium was almost 2 times that of ischem ic myocardium. Conclusion: Insulin stimulation results in GLUT4 translocation and increases glucose uptake in ischemic myocardium. When myocardi al ischemia occurs, insulin is helpful in increasing myocardial glucose uptake a nd utilization.
