Effect of antisense transfection of monocarboxylate transporter on cell biological characteristics in human lung adenocarcinoma cells
- VernacularTitle:单羧酸转运蛋白反义基因转染人肺腺癌细胞对其生物学特性的影响
- Author:
Guizhi ZHANG
;
Guijun HUANG
;
Xianjian GUO
;
Guisheng QIAN
- Publication Type:Journal Article
- From:Journal of Third Military Medical University
2001;23(2):128-130
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effect of transfecting anti-sense expression vector of the first subtype of the monocarboxylate transporter (MCT1) gene into human lung adenocarcinoma cells on intracellular pH (pHi) regulation, lactate transportation and cell growth. Methods MCT1 antisense gene recombinant vector pLXSN-MCT1 was introduced into human lung cancer cells A549 with electroporation. The cell colonies resistant to G418 were selected. Positive clones were examined by PCR to confirm the integration of genomic A549 DNA and antisene MCT1 gene. The changes of pHi and lactate transportation were detected with spectrophotometry. Cell growth was studied with cell growth curve. Results pHi and lactate transport were remarkably decreased in the transfected cells, and the cell growth was inhibited compared with the cells without transfection（P<0.001）. Conclusion MCT1 gene may play an important role in pHi regulation, lactate transport and cell growth in lung tumor cells.
