Establishment of a multidrug resistance cell line from human lung adenocarcinoma cells and its biologic features
- VernacularTitle:人肺腺癌多药耐药细胞系的建立及其生物学特征
- Author:
Jie CHEN
;
Guisheng QIAN
;
Guijun HUANG
;
Wei XIONG
- Publication Type:Journal Article
- From:Journal of Third Military Medical University
2001;23(2):135-137
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a multidrug resistance cell line from human lung adenocarcinoma. Methods The human lung adenocarcinoma cell line SPC-A-1 was exposed to cisplatin of a high and then increasing concentration for 192 d to establish multidrug resistance cell line (SPC-A-1/CDDP). The relative resistance was tested with MTT assay. The morphology of the cells was observed with transmission and scanning electron microscopy and the chromosome of them was analyzed with Giemsa stained specimens. Results The resistance index of SPC-A-1/CDDP cells to cisplatin was 11.2 and the cells showed various cross-resistance to 5-Fu, doxorubicin, mitomycin, vincristine and etoposide, but not to hydroxycamptothecine. Electron microscopy showed the cells with irregular and enlarged nuclei and abundant microvilli. Conclusion A multidrug resistance cell line (SPC-A-1/CDDP) from human lung adenocarcinoma is established. It can be used to downstream experiment.
