Application of monoclonal antibody pool against Bacteroides fragilis and Clostridium perfringens in rapid diagnosis in surgical infection
- VernacularTitle:抗脆弱类杆菌和产气荚膜杆菌McAb池在外科感染快速诊断中的应用
- Author:
Yaping ZHANG
;
Lu GAN
;
Shan CHANG
- Publication Type:Journal Article
- From:Journal of Third Military Medical University
2001;23(2):224-226
- CountryChina
- Language:Chinese
-
Abstract:
Objective To prepare monoclonal antibody (McAb) pool against Bacteroides fragilis and Clostridium perfringens and evaluated its effect in rapid diagnosis in surgical infection. Methods After the preparation of monoclonal antibodies against B. fragilis and C. perfringens, 191 specimens collected from infectious patients was detected with indirect immunofluorescent antibody (IFA) and enzyme labeled antibody (ELA). The results were compared with routine cultural method (CM). Results Among the 191 specimens, 53 (27.7%), 55 (28.8%) and 25 (13.1%)strains of B. fragilis were detected with IFA, ELA and CM respectively, and for C.perfringens, 12(6.3%), 11(5.8%), and 6(3.1%) strains were found. The detectable rate of anaerobic bacteria by IFA and ELA was higher than by CM, but no difference was found between these 2. Conclusion The self-made McAb pool against B.fagilis and C.perfringens might be a rapid, specific, sensitive and simple McAb pool for detecting B.fragilis in surgical infection and C.perfringens in wartime.
