Screen-Printing Enzyme Electrode-Immobilizing the Enzyme with Silk Fibroin

VernacularTitle:丝素膜固定化丝网印刷酶电极
Author: Yifeng TU ; Zhiqiang FU ; Mingzhong LI
Publication Type:Journal Article
From: Chinese Journal of Analytical Chemistry 2001;29(2):165-167
CountryChina
Language:Chinese
Abstract: This paper reports the technique to develop a disposable screen-printing base carbon electrode for the utilization in the study of enzyme electrode. The silk fibroin was applied to immobilize the mushroom extracted protein, which contained abundant polyphenol oxidase, on the surface of base electrode when it was chemically moldified by ferrocene. The voltammetric current of this enzyme electrode responded to the concentration of substrate such as catechol or dopamine. The linear range is from 2.0× 10-8 mol/L to 2.0× 10-2 mol/L, it reaches to a 95%steady value of current within less than 30 seconds, and the RSD equals to 2.7%. The service life of this enzyme electrode is at least 10 days and it will be preserved for a longer period at a humid and cool condition. All of these performances of the enzyme electrode make clear that its commercial development would be very possible.