Characteristics of Molecular Recognition of Norfloxacin Template Polymer
- VernacularTitle:药物氟哌酸分子模板聚合物的分子识别特性
- Author:
Hongsheng GUO
;
Xiwen HE
;
Jie ZHOU
;
Hong LIANG
- Publication Type:Journal Article
- From:
Chinese Journal of Analytical Chemistry
2001;29(2):128-132
- CountryChina
- Language:Chinese
-
Abstract:
A molecular template synthetic polymer highly selective for norfloxacinum was prepared by a molecular imprinting technique. The selective binding characteristics of the template polymer was evaluated by Scatchard analysis. The multiple-sites binding model was used to calculate the maximum numher of binding sites and the dissociation constant. The results showed that the template polymer using methacrylic acid (MAA) as functional monomer could form two kinds of binding sites. The dissociation constants were estimated to be Kd1 = 2.9 × 10-5mol/L and Kd2 = 3.2 × 10-3 mol/L. The selective binding experiment for substrates indicated that the polymer gave much higher affinity and selectivity for norfloxacin than for acid pipemidic and cefalexin. It is possible to be a good adsorption and binding material in the selective enrichment and determination of trace norfloxacin in complex biosamples.
