Nuclear translocation of apoptosis-related protein TFAR19 in TF-1 cells undergoing apoptosis
- VernacularTitle:凋亡相关蛋白TFAR19在TF-1细胞凋亡中出现细胞核转位
- Author:
Yingyu CHEN
;
Ronghua SUN
;
Wenling HAN
;
Yingmei ZHANG
;
Quansheng SONG
;
Chunhui DI
;
Dalong MA
- Publication Type:Journal Article
- From:
Journal of Peking University(Health Sciences)
2001;33(2):97-100
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To study the expression and localization of apoptosis-related protein TFAR19 in TF-1 cells undergoing apoptosis. Methods: Using monoclonal antibody against TFAR19, the expression level and cell localization of TFAR19 were examined by fluorescence microscope, confocal laser scan microscope(CLSM) and flow cytometry. Simultaneously, we also analyzed the relationship of TFAR19 protein with phosphatidylserine (PS) externalization and cell nuclear DNA fragmentation. Results: The level of TFAR19 proteins expressed in TF-1 cells treated with GM-CSF withdrawal was significantly increased compared with normal TF-1 cells, then translocated rapidly from cytoplasm to the nucleus of cells. Appearance of TFAR19 in the nucleus of apoptotic cells preceded the detection of PS externalization and DNA fragmentation. Conclusion: Nuclear translocation of TFAR19 protein is one of the earliest events of cell apoptotic process. These data provided a new clue to further approach to the biological function of TFAR19 and study of cell apoptosis.
