Modification of GABAA receptor subunit mRNA content in audiogenic seizure rat cortex and hippocampus following tolerance to flurazepam
- VernacularTitle:氟西泮耐受听源性惊厥大鼠脑皮质运动区和海马区GABAA受体亚单位mRNA含量的变化
- Author:
Wen GUO
;
Li WANG
- Publication Type:Journal Article
- From:
Journal of Peking University(Health Sciences)
2001;33(2):105-108
- CountryChina
- Language:Chinese
-
Abstract:
Objective： To investigate the modification of GABAA receptor subunits in audiogenic seizure rat cortex and hippocampus when rendered tolerant to flurazepam. Methods： Rats were administrated with flurazepam for two weeks, which resulted in tolerance to flurazepam, in the absence of behavioral signs of withdrawal. GABAA receptor subunits α1, α3, α5, γ2L and γ2S were assayed using quantitative competitive RT-PCR in rat frontparietal motor(FrPaM) and hippocampus. Results： In FrPaM, the contents of mRNA encoding for α1, α3, γ2L and γ2S were all significantly decreased (24%,17%,35% and 45% respectively),whereas that of α5 was significantly increased (33%) compared with the control. In hippocampus, α1, γ2L, and γ2S mRNA contents were significantly decreased (33%, 35%and 27% respectively). Conclusion： The accomodated change in GABAA receptor subunits α1, α3, α5,γ2L and γ2S in FrPaM and hippocampus may be associated with the mechanism of flurazepam tolerance in audiogenic seizure rats.
