Effect of cyclical mechanical force on expression of mRNAs for osteoblast-like marker proteins in human periodontal ligament cells in vitro

VernacularTitle:周期性牵张力对人牙周膜细胞中成骨样细胞表型碱性磷酸酶和骨钙素mRNA表达的影响
Author: Ding ZHANG ; Xiaotong LI ; Minkui FU
Publication Type:Journal Article
From: Journal of Peking University(Health Sciences) 2001;33(2):118-121
CountryChina
Language:Chinese
Abstract: Objective： To study the role of mechanical stress on osteoblast-like characteristics expressed by human periodontal ligament cells in vitro. Methods： Human periodontal ligament cells (hPDLC) were cultured by explant method. We investigated the gene expression for osteoblast-like marker proteins in periodontal ligament stimulated by cyclical mechanical force in vitro by in situ hybridization with non-radioisotopic oligonucleotide probe for alkline phosphatase (ALP) and osteocalcin (OCN). Results： Human periodontal ligament cells exhibited a stronger signal for ALP mRNA stimulated by cyclical mechanical force, and exhibited a moderately intense positive signal for OCN mRNA—OCN was a marker of differentiated osteoblast-like cells. Conclusion： These findings suggest that mechanical force plays role in differentiation from hPDLC to osteoblast-like cells.