Helicobacter pylori induces the synthesis of cyclooxygenase-2 in human gastric epithelial carcinoma cell line BGC-823
- VernacularTitle:幽门螺杆菌诱导人胃上皮癌细胞株BGC-823环加氧酶-2的合成
- Author:
Yongchang CHEN
;
Jianzhong ZHANG
;
Tie LI
;
Tao SHENG
;
Wenyu ZHU
- Publication Type:Journal Article
- From:
Journal of Peking University(Health Sciences)
2001;33(2):144-146
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the effect of Helicobacter pylori (H. pylori) on the synthesis of cyclooxygenase-1 (COX-1) and cyclooxygenase-2 (COX-2) in gastric epithelial cells. Methods: a VacA(+) and CagA(+) international standard H. pylori line NCTC11637 and a human gastric epithelial carcinoma cell line BGC-823 were used. Western Blotting was applied to detect the synthesis of cyclooxygenase. Results: The content of COX-2 protein increased obviously after the cells were incubated with H. pylori sonicating extract for 1 h and the increase lasted for at least 6 h whereas the content of COX-1 protein did not change during the incubation with H. pylori extract. H. pylori lipopolysaccharide (LPS) had no effect on COX-2 synthesis. Conclusion: H. pylori stimulated the synthesis of COX-2 in BGC-823 cells and the effect was LPS-independent.
