Construction of recombinant baculovirus vector of HIV-1 gag-gp120 chimeric gene
- VernacularTitle:HIV-1 gag-gp120嵌合基因重组杆状病毒载体的构建
- Author:
Dongwei ZHANG
;
Ningyi JING
;
Hongwei WANG
;
Yingjiu ZHANG
;
Lixin WANG
;
Kun LUO
;
Ping LI
;
Hongchen YU
;
Zhen YIN
- Publication Type:Journal Article
- From:
Journal of Jilin University(Medicine Edition)
2001;27(2):114-116
- CountryChina
- Language:Chinese
-
Abstract:
Objective:A new recombinant baculovirus transfer vector was constructed, in whic h a recombinant gene fragment encoding HIV-1 gag-gp120 chimeric gene was inse rted.Methods:After HIV-1 gag gene and gp120 gene were linked,the recombinant baculov irus vector was constructed,and the DNA recombinant technique and the E coli /baculovirus system were used.Results:Gel electrophoresis of DNA analysis showed that the genes were recombine d correctly.Electron microscope showed that the recombinant baculovirus reproduc ed in a great quantity.Conclusion:Recombinant baculovirus vector which HIV-1 gag-gp120 chimeric gene fragment was inserted in was constructed successfully.This vector is useful in study of the expression and the biological function of the HIV-1 Gag-gp120 ch imeric protein.
