Oxidative modification of rat β2-glycoprotein Ⅰ
- VernacularTitle:大鼠β2-糖蛋白Ⅰ的氧化修饰
- Author:
Tong SHI
;
Xueli WANG
;
Like QU
;
Hanyi YANG
- Publication Type:Journal Article
- From:
Journal of Jilin University(Medicine Edition)
2001;27(2):117-118
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate oxidative modification of β2-glycoprotein Ⅰ in vit ro.Methods:Rat β2-glycoprotein Ⅰ was purified and characterized,then oxidize d by hypoxanthine plus xanthine oxidase as a supreroxide free radical generating system;carbonl groups of β2-glycoprotein Ⅰ were detected by the reaction w ith 2,4-dinitrophenylhudrazine.Results:There was a significant increase of carbonyl groups formation in β2- glycoprotein Ⅰ oxidized in comparison with native β2-glycoprotein Ⅰ (P <0.05). Conclusion:Carbonyl groups have been formed in vitro on rat β2-glycoprotein Ⅰ after oxidative modification using hypoxanthine plus xanthine oxidase system.
