Breeding of highly productive γ-linolenic acid mutant strain of Mortierella ramanniance
- VernacularTitle:拉曼被孢霉γ-亚麻酸高产突变株的选育
- Author:
Zhifeng LI
;
Ling ZHANG
;
Bingsen LAI
;
Xiaojing SHEN
;
Yafang TAN
;
Shuqin SUN
- Publication Type:Journal Article
- From:
Chinese Journal of Biochemical Pharmaceutics
2001;22(3):129-131
- CountryChina
- Language:Chinese
-
Abstract:
Purpose The aim is to breed a highly productive γ-linolenic acid mutant strain.Methods The original strain Mortierella ramanniance AS 3.3413 was treated by 5-FU,UV,LiCl as mutanting agent.Results A highly productive γ-linolenic acid mutant strain F5 was successfully obtained.The γ-linolenic acid yield of mutant strain F5 was increased by 300% as compared with that of the original strain.Pass-generation test showed that the heredity character of mutant strain F5 was stable.After the optimal medium and culture condition was established, the yield of γ-linolenic acid was up to 1 153.63 mg/L,and was increased by 335.87% as compared with that of the original strain.Conclusion The mutant strain can be used for the industrial production of GLA.
