Study on immobilization and stability of thrombin
- VernacularTitle:凝血酶的固定化及其稳定性研究
- Author:
Ping LI
;
Shuling REN
;
Yi ZHANG
;
Ping WEI
- Publication Type:Journal Article
- From:
Chinese Journal of Biochemical Pharmaceutics
2001;22(3):134-136
- CountryChina
- Language:Chinese
-
Abstract:
Purpose The aim is to immobilize the thrombin and to increase the stability of immobilized enzyme.Methods Thrombin was immobilized on chitosan and crosslinked with glutaraldehyde, and the optimum conditions for immobilization and stability of immobilized enzyme were studied.Results This preparation of immobilized thrombin was practicable,and the stability of this immobilized enzyme was obviously improved than that of thrombin.Conclution The conditions for immobilization were good, and reliable.Stability of immobilized thrombin to light, high temperature,low temperature and observation on reserving at room temperature was good.
