Initial experimental research on placenta extract to stimulate lymphocyte proliferation activity and stability
- VernacularTitle:胎盘提取物促淋巴细胞增殖活性及稳定性实验初探
- Author:
Guohua WANG
;
Wangtai CHENG
;
Hongyan MA
;
Zhiyang CHEN
;
Libo YAO
- Publication Type:Journal Article
- From:
Chinese Journal of Biochemical Pharmaceutics
2001;22(3):137-139
- CountryChina
- Language:Chinese
-
Abstract:
Purpose The aim is to prepare the extract of immunocompetent cell proliferation from human placenta and to try to find out a suitable method of preserving the extract.Methods The extract was mainly prepared by heat-treating placenta homogenated fluid. Then the activity to stimulate murine splenic lymphocyte proliferation in vitro was done with MTT , exposing the extract to radioisotope 60 Co.Results The content of protein was 2～3mg per gram placenta measured by Lowry′s Method.The rate to promote cell proliferation was more than 80 percent.The activity lasted a few months after being exposed to radioisotope.Conclusion The extract prepared by heat-treating not only had high activity, but also had the unique method and good repeatability.This prepared extract as a kind of stable,reliable and remarkably promoting lymphocyte proliferation reaction had the value of development production on broad scale as well as in clinical practice.
