Microwave assisted extraction of superoxide dismutase from pig blood
- VernacularTitle:微波加热破膜提取猪血超氧化物歧化酶的研究
- Author:
Zhilong XIU
;
Wei WANG
;
Meiqing LU
- Publication Type:Journal Article
- From:
Chinese Journal of Biochemical Pharmaceutics
2001;22(3):139-141
- CountryChina
- Language:Chinese
-
Abstract:
Purpose The aim is to establish a simple method to extract superoxide dismutase(SOD) from pig blood red cells.Methods A combined method-microwave heating and adding Cu2+ into the suspension-was used to extract SOD from the red cells. Results The undesired proteins were denatured by microwave heating and SOD was partly purificated in comparison with hemolysis. The processing time of the former was much shorter than the latter. Conclusion A novel cell disruption microwave heating, was a rapid and effective technique for the primary extraction of SOD.
