Study on the Preparation Technic of Pheophrobide From Silkworm Excrement
- VernacularTitle:从蚕砂中制备脱镁叶绿酸的工艺研究
- Author:
Zhangyang WANG
;
Ying ZHOU
- Publication Type:Journal Article
- From:
China Pharmacy
2001;12(3):148-149
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To optimize the factors of preparing pheophrobide from silkworm excrement and raise the yield of pheophrobide.METHODS:Based on the extraction rate of chlorophyll,acetone method was compared with alcohol method and the preparing factors of acetone method was optimized by orthogonal design.RESULTS:The best conditions for pheophrobide preparation included grinding silkworm excrement into powder with particle size all below 250μ m,softening the powder more then 5h with water 1/10 the volume of powder,extracting the powder with acetone three times the volume of powder at 70℃ for two times 60min each,regulating pH of the liquid to 2.5 with HCl solution.CONCLUSION:The water and acetone volume was more important than other factors,the yield of pheophrobide was raised 3.6 times after optimization
