Determination of Serum Concentrations of Propofol by RP－ HPLC
- VernacularTitle:反相高效液相色谱法测定异丙酚血清浓度
- Author:
Shuomin JIANG
;
Zengshou WANG
;
Shengwei JIN
- Publication Type:Journal Article
- From:
China Pharmacy
2001;12(3):161-162
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE： A RP－ HPLC method was established for determination of serum concentration of propofol in man METHODS： The mobile phase consisted of methanol－ water（ 68∶ 32v/v） The detection was carried out at wave length 258nm and flow rate 1ml/min with carbamazepine as internal standard RESULTS： The retention times of propofol and carbamazepine were 9 20 and 5 16 min， respectively The mean recovery of propofol was 99％ The within－ day and inter－ day variations were all less than 10％ Propofol and carbamazepine were seperated well The assay linearity was obtained in the range of 1～ 16μ g/ml in serum（ r=0 9 994） CONCLUSION： The method is sensitive， simple and reliable for the determination of propofol concentration
