Expression of BMP and TGF-β1 in Regenerative Gap During Tibial Lengthening by Metaphyseal Osteotomy of Rabbits
- VernacularTitle:兔胫骨干骺端截骨延长延长区BMP、TGF-β1的蛋白表达
- Author:
Fei DAI
;
Meiying WU
;
Qihong LI
- Publication Type:Journal Article
- From:
Orthopedic Journal of China
2001;8(3):265-267
- CountryChina
- Language:Chinese
-
Abstract:
To study the expression of bone morphogetic protein(BMP) and transforming growth factor β１（TGF-β１) in regenerative gap during lengthening period. Method: The right tibia of 14 mature rabbits were lengthened using metaphyseal osteotomy. Distraction was started on the 4th day postoperatively, 1mm per day and conducted twice each day. The expression of endogenesis BMP and TGF-β１ in regeneraive gap were observed during ten weeks after distraction by histology and immunohistochemisty. Results: The result showed that bone morphogetic protein(BMP) and transfoming growth factor β１(TGF-β１) were expressed in chondrocytes, osteoblasts, and fibroblasts. The expression of BMP was at a high level in initial two weeks after distraction while the expression of TGF-β１ was increased step by step. Then both of them decreased alonged with lengthening proceeding. Two weeks after distracting stopped, the expression of BMP could not being observed. But the expression of TGF-β１ could being observed all stage after distraction stopped. Conclusion: BMP and TGF-β１ were expressed persistently in regenerative gap during bone lengthening.
