ffect of basic fibroblast growth factor on rabbit lens epithelial cell proliferation
- VernacularTitle:碱性成纤维细胞生长因子对兔晶状体上皮细胞增殖的影响
- Author:
Hui PENG
;
Pinghua LI
;
Lixia LUO
;
Yunpeng LUO
;
Weixue TANG
- Publication Type:Journal Article
- Keywords:
basic fibroblast growth factor;
rabbit lens epithelial cells;
cell proliferation
- From:
Recent Advances in Ophthalmology
2001;21(3):161-163
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effect of basic fibroblast growth factor (bFGF) on proliferating of rabbit lens epithelial cells(RLECs).Methods The second and three generations of RLECs were exposed to different concentrations of bFGF, and the proliferation characteristics of the cells were measured with methyl thiazolyl tetrazolium (MTT) assay. The ultrastructure of cell were observed with transmission electron microscope (TEM).The changs of cells cycles were observed with flow cytometer (FCM).Results After the treatment of bFGF, RLECs showed the marked proliferation , especially in 10μg*L-1 of bFGF . TEM result showed that the cells were more active with bFGF, FCM result showed that the S phase cell obviously increased.Conclusion bFGF is an important factor that can promote proliferation of RLECs.
