STUDIES ON THE ACTIVE POLYSACCHARIDES FROM LYCIUM BARBARUM L.
- VernacularTitle:枸杞子活性多糖的研究
- Author:
Changling DUAN
;
Naili WANG
;
Xinsheng YAO
;
Shanyi QIAO
;
Yimin ZHAO
;
Chunhui QI
- Publication Type:Journal Article
- From:
Acta Pharmaceutica Sinica
2001;36(3):196-199
- CountryChina
- Language:Chinese
-
Abstract:
AIM To investigate the structures and immunomodulation activity of four homogeneous polysaccharides: LBP 1a-1, LBP 1a-2, LBP 3a-1 and LBP 3a-2 isolated from Lycium barbarum L. brought from Zhongning County, Ningxia Province. METHODS Their molecular weights, sugar component (constituents) and their linkages were determined by gel permeation chromatography, acid hydrolysis, periodate oxidation and NMR spectrum. The activity of immunomodulation was evaluated with splenocyte proliferation by ［3H］-TDR incorperation, in vitro. RESULTS Four polysaccharides with molecular weights 11.5×104, 9.4×104, 10.3×104 and 8.2×104, were shown to enhance splenocyte proliferation induced by ConA. LBP 1a-1 and LBP 1a-2 were α-(1→6)-D-glucans. LBP 3a-1 and LBP 3a-2 were found to be α-(1→4)-D-polygalacturonans. CONCLUSION The four polysaccharides were first isolated from this plant. Polysaccharides with main chain of α-(1→4)-D-polygalacturonans showed stronger immunomodulation activity.
