STUDY ON THE PHASE I METABOLITES OF PHONEPROLAMINE HYDROCHLORIDE IN RAT BILE BY LC/DAD/MSD
- VernacularTitle:LC/DAD/MSD技术研究大鼠服药胆汁中盐酸非洛普I相代谢产物
- Author:
Li DING
;
Zhengxing ZHANG
;
Dengkui AN
;
Peizhou NI
;
Guangji WANG
- Publication Type:Journal Article
- From:
Acta Pharmaceutica Sinica
2001;36(3):205-209
- CountryChina
- Language:Chinese
-
Abstract:
AIM To study the phase I metabolites of phenoprolamine hydrochloride (DDPH) in rat bile. METHODS DDPH was administered ip to bile duct-cannulated rats. Bile samples were collected before administration and up to 12 h after administration. After being treated with β-glucuronidase, the bile samples were purified and enriched with C-18 SPE columns, and then were analyzed by LC/DAD/MSD. The samples containing synthesized reference standards of DDPH metabolite 1-(2,6-dimethylphenoxy)-2-(3-methoxy-4-hydroxyphenylethylamino)-propane (M1), 1-(2,6-dimethyl-3-hydroxyphenoxy)-2-(3,4-methoxy-phenylethylamino)-propane (M2), 1-(2,6-dimethyl-4-hydroxyphenoxy)-2-(3,4-methoxyphenylethylamino)-propane (M3), 1-(2,6-dimethyl-4-hydroxyphenoxy)-2-(3-hydroxy-4-methoxyphenylethylamino)-propane (M4), 1-(2,6-dimethyl-3-hydroxyphenoxy)-2-(3-hydroxy-4-methoxyphenylethylamino)-propane (M5) and 1-(2,6-dimethyl-4-hydroxyphenoxy)-2-(3-methoxy-4-hydroxyphenylethylamino)-propane (M6) were analyzed by LC/DAD/MSD under identical conditions. RESULTS The retention times, UV spectra, molecular weights and production spectra (obtained by collision-induced dissociation)of the apparent ions of peak A, B, C, D, E and F in the total ion chromatogram of DDPH treated rat bile sample were consistent with those of M1, M2, M3, M5, M4 and M6, respectively. CONCLUSION M1, M2, M3, M4, M5 and M6 were identified as the phase I metabolites of DDPH in the rat.
