Application of HBeAg produced in prokaryotic cells and eukaryotic cells
- VernacularTitle:原核和真核细胞表达HBeAg的应用研究
- Author:
Liang HE
;
Xiaozhao DENG
;
Zhenyu DIAO
;
Zongan ZHOU
;
Jishan ZHENG
;
Jian GAO
- Publication Type:Journal Article
- From:
Journal of Medical Postgraduates
2001;14(3):204-206
- CountryChina
- Language:Chinese
-
Abstract:
Objectives: To express HBeAg in prokaryotic and eukaryotic cells and compare the two types of HBeAg in the anti-HBeAg testing. Methods: HBeAg was expressed both in E.coli cells and in silk worm cells, purified by Sephacryl S-200.HBeAg protein concentration and antigenic titer were determined respectively by ultraviolet-spectroscopy and EIA. Results: HBeAg produced by E.coli cells: Activation ratio was 10 000/mg, HBeAg/HBcAg = 50; The specificity in testing anti-HbeAg was 96%;HBeAg produced by silk worm cells: Activation ratio was 160 000/mg, HBeAg/HBcAg = 5 000, The specificity in testing anti-HbeAg was 100%. Conclusions: HBeAg produced by eukaryotic cells contained much lower proportion of HbcAg and higher activation ratio, which therefore bring about a possibility to improve the quality of the kit for testing Anti-HBe.
