Study of β-defensin rBD-2 gene expression in the pulmonary tissues of the fetal, neonatal and adult rats
- VernacularTitle:大鼠不同发育阶段β-防御素-2基因在肺组织的表达研究
- Author:
Hui ZHOU
;
Ning HUANG
;
Xinnian CHEN
;
Qi WU
;
Boyao WANG
- Publication Type:Journal Article
- From:
Chinese Journal of Pathophysiology
2001;17(3):226-229
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To investigate the developmental regulation of β-defensin rBD-2 gene expression in the rat lung. METHOD: Total RNA was isolated from the pulmonary tissues of the fetal, neonatal and adult rats. RT-PCR were performed with primers (P1: TTCAGTCATGAGGATCCATT AC; P2: TGGAACTTGGTCTTTTTATCTAC). The RT-PCR products were cloned into pGEM-T easy vector and the recombinant plasmid was analyzed with EcoR1 digestion and the inserted DNA sequencing was performed on ABI PRISM-377 DNA sequencer. RESULTS： Rat β-defensin-2 transcripts were detected in all the pulmonary tissues of rats during different developmental stages, e.g. at just before birth, 8 hours and 4 days after birth , and adult. CONCLUSION： The rat β-defensin-2 is constitutively expressed in the pulmonary tissues, suggesting that β-defensin-2 may play a role in the lung innate defense against infection.
