The effect of microelement powder on membrane potential of vascular endothelial and smooth muscle cells of rats
- VernacularTitle:微元素粉末对大鼠血管内皮和平滑肌细胞膜电位的影响
- Author:
Jie LIU
;
Xuliang HUANG
;
Yiman WU
;
Qiaobing HUANG
;
Kesen ZHAO
- Publication Type:Journal Article
- From:
Chinese Journal of Pathophysiology
2001;17(3):235-237
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To study the effect of microelement powder (MP) on membrane potential of vascular endothelial and smooth muscle cells of rats in order to elucidate the mechanism of microcirculation improvement by MP. METHODS: Cultured pulmonary vascular endothelial cells (EC) and aortic smooth muscle cells (SMC) of rats and detecting the changes of cellular membrane potentials by using potential-sensitive fluorescent probe and laser jet confocal microscope. RESULTS: MP hyperpolarized SMCs significantly. Glybenclamide (2 μmol/L), a blocker of KATP channel, which had no effect on membrane potential of SMCs, reversed the hyperpolarization of MP completely; MP hyperpolarized ECs slightly, but the effect was unaffected by glybenclamide. CONCLUSION: MP hyperpolarizes SMCs by activating KATP channels and leads to dilation of microvessels and improvement of microcirculation.
