Brain damage of cerebral ischemia/reperfusion and expression of TGF-β1 mRNA in diabetic rats
- VernacularTitle:糖尿病大鼠局灶脑缺血/再灌注损伤与脑组织TGF-β1 mRNA表达
- Author:
Guixia WANG
;
Guangren LI
;
Tongshu YANG
;
Shao WANG
- Publication Type:Journal Article
- From:
Chinese Journal of Pathophysiology
2001;17(3):259-263
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To observe the difference of cerebral injury following ischemia/reperfusion and mRNA expression of TGF-β1 between diabetic and non-diabetic rats.METHODS: At first, Wistar rats were divided into two groups,non-diabetes and diabetes, and then two groups followed by sham, middle cerebral artery occlusion(MCAO) 2h and reperfusion 24 h after MCAO 2 h respectively. TGF-β1 mRNA expression was measured by semi-quantitative reverse transcription polymerase chain reaction(RT-PCR); Cerebral damage was evaluated by histopathology.RESULTS: In the same condition of ischemia or ischemia/reperfusion, injuried area enlarged in DM groups; The expression level of TGF-β1 mRNA increased at the time of 2 h after MCAO in non-diabetic group and diabetic group, especialy significantly in non-diabetic group with MCAO 2 h, and decreased at the time of reperfusion 24 h after MCAO 2 h, but still higher than that in the sham group.CONCLUSION: Diabetes mellitus exacerbated brain lesion following ischemia/repefusion, increased TGF-β1 mRNA expresion after MCAO may be an anti-injury reaction,and anti-injury ability is decreased under diabetic condition
