The effect of vasoactive substances on synthesis and release of C-type natriuretic peptide in cultured human endothelial cells
- VernacularTitle:血管活性物质对人内皮细胞C-型利钠利尿肽合成和释放的影响
- Author:
Xiaohong WANG
;
Yahong CHEN
;
Yongfen QI
;
Shulian LI
;
Yongzheng PANG
;
Naikui LIU
;
Chaoshu TANG
- Publication Type:Journal Article
- From:
Chinese Journal of Pathophysiology
2001;17(3):193-195
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To investigate the effect of endothelin (ET), angiotensin II (AngII) and homocysteine (Hcy) on C-type natriuretic peptide (CNP) synthesis and release. METHODS: Human endothelial cell was cultured; CNP was measured by radioimmunoassay method. RESULTS: ET and AngII could augment CNP synthesis in human endothelial cells. Compared with control group, 10-9,10-8,10-7 mol/L ET and Ang II increased CNP content of endothelial cells by 1%(P＞0.05), 49%(P＜0.05),117%(P＜0.01) and 137% (P＜0.01),165%(P＜0.01),201%(P＜0.01),respectively. A great dose of ET and Ang II also stimulated CNP release from cultured human endothelial cells. Hcy had no effect on CNP synthesis, but 10-9，10-8,10-7 mol/L Hcy enhanced CNP release from cultured human endothelial cells by 17%(P＞0.05),84%(P＜0.01) and 555%(P＜0.01), respectively. CONCLUSION: ET, AngII and Hcy might be involved in the synthesis and release of human endothelial cell CNP.
