The primordial study of application of primer dimer in the gene clone
- VernacularTitle:引物二聚体在目的基因克隆中的应用初探
- Author:
Xiang XU
;
Xin LIU
;
Huaping LIANG
;
Jiaping DAI
;
Qiongguo MAO
;
Yan LUO
- Publication Type:Journal Article
- From:
Chinese Journal of Clinical Laboratory Science
2001;19(3):135-136
- CountryChina
- Language:Chinese
-
Abstract:
Objective According to principle of forming primer dimer,a new cloning gene method was established. Methods Two and more oligonucleotide with complementary partnership in the 3′ end were synthesized by artifical method,oligonucleotide,Taq DNA polymerase and dNTP were mixed in the proportion of reasonable,then expanded and extended by PCR,result was identifed by agarose gel electrophoresis.Results A clear special band was appeared in the agarose gel. Conclusion Short fragment target gene less than 500 bp may be synthesized by this method.
