Establishment and clinical application of quantitative RT-PCR method for detecting perforin mRNA
- VernacularTitle:定量RT-PCR检测穿孔素mRNA方法的建立与临床初步应用
- Author:
Weisong QIN
;
Fangqiu LI
;
Jianguo WU
- Publication Type:Journal Article
- From:
Chinese Journal of Clinical Laboratory Science
2001;19(3):137-139
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish the quantitative method for detecting perforin mRNA. Methods The competitive templates were prepared by restriction endonuclease method.The quantitative RT-PCR assay was established and used to detecting the perforin mRNA level in peripheral blood of tumor patients and healthy adults.Results Average perforin mRNA level of six healthy adults is 0.51±0.40 pmol/ml.The perforin mRNA level of patients with digestive tumors are significantly lower than that of healthy adults(P<0.01).But compared with healthy adults,no significant difference is showed in patients with breast cancer or leukemia.Conclusion The decreased perforin mRNA level probably attributes to the high incidence of cancers.Except for perforin-mediated cytolysis,additional effective mechanism against tumor cells maybe exist.
