Mutations of P16 Gene in 8 Human Malignant Lymphoma Cell Lines
- VernacularTitle:8种人恶性淋巴瘤细胞系P16基因突变的研究
- Author:
Nianlan YANG
;
Yan CHEN
;
Chongyu LI
- Publication Type:Journal Article
- From:
Acta Medicinae Universitatis Scientiae et Technologiae Huazhong
2001;30(3):229-231
- CountryChina
- Language:Chinese
-
Abstract:
In order to determine the relationship between P16 and the tumorigenesis of malignant lymphoma，silver-stained PCR-SSCP analysis and direct sequencing were used to evaluate the alterations of P16 gene in 8 human malignant lymphoma cell lines. A homolygous loss was found in SU-DHL-9 while mutations of P16 exon 2 in SU-DHL-1 and Daudi. Sequencing of the PCR-product showed three point mutations in SU-DHL-1，which were GAC to AAC in codon 72，GOT to ACT in codon 77，and a insertion of C between codon 82 and 83，respectively. This study suggested the mutations of P16 gene are highly frequent in B-cell lymphoma cell lines and may play an important role in its tumorigenesis.
