The Clinical Use of Double-paper Synergy Test in the Detection of Bacteria Producing Extended-spectrum β-lactamase
- VernacularTitle:双纸片协同试验在检测产超广谱β-内酰胺酶细菌中的临床应用
- Author:
Wei XIONG
- Publication Type:Journal Article
- From:
Acta Medicinae Universitatis Scientiae et Technologiae Huazhong
2001;30(3):285-288
- CountryChina
- Language:Chinese
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Abstract:
The optimal condition of double-paper synergy test in the detection of bacteria producing extended-spectrum β-lactamase (ESBLS) was studied. In 244 isolates of Enteric bacilli，Klebsiella pneumonia，Enteric cloacae，there were 49 ESBLS-producing strains with the positive rate being 19.67 %. Five ESBLS-producing strains were detected in Pseudomonas maltophilla. One ESBLS-producing strains was detected in Pseudomonas aeruginose，Enteric aerogenes and Citrobacter，respectively. The optimal substrates of double-paper synergy test were cefotaxime，aztreonam and cefepime，and the interspace was 25 mm. This method was still a simple，economical and appropriate method in the detection of ESBLS-producing isolates.
