Separation and Determination of Cinoxacin and Nalidixic Acid inHuman Plasma by Reverse-phase High Performance Liquid Chromatography
- VernacularTitle:反相高效液相色谱法同时测定人血浆中的西诺沙星和萘啶酸
- Author:
Hua DENG
;
Haixia ZHANG
;
Jingsong ZHANG
;
Mancang LIU
- Publication Type:Journal Article
- From:
Chinese Journal of Analytical Chemistry
2001;29(3):306-308
- CountryChina
- Language:Chinese
-
Abstract:
A method for the determination of cinoxacin and nalidixic acid in human plasma by reverse-phase high performance liquid chromatography is introduced. The single separation system involves a RP-C18 column, a ultraviolet detector at 254 mn and CH3CN-CH3OH-0.01 mol/L HOOCOOH (30:5:65;pH 4.55). The influence of two kind of buffer (phosphate buffer and oxalate buffer) in mobile phase on the separation were studied. Oxalate buffer can improve tailing peak and decrease detection limits. Three pH values (pH 2.80,4.08,4.55) were studied, pH 4.55 can give best separation result. The detection limits of cinoxacin and nalidixic acid were 0.14 ng and 0.24 ng, respectively.
