A Phosphorimetric Method for Determination of Folic Acid
- VernacularTitle:叶酸的低温磷光分析
- Author:
Ruheng HUANG
- Publication Type:Journal Article
- From:
Chinese Journal of Analytical Chemistry
2001;29(3):317-319
- CountryChina
- Language:Chinese
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Abstract:
The phosphorescence intensity of folic acid is stronger in acidic medium (340/450 nm) than that of in alkaline medium (390/520 nm). As the concentration of HCl increasing. the phosphorescence intensity become stronger, but very unstable on standing. The phosphorescence increases 20 times after UV irradiation in 0.1 mol/L HCl. The increment of phosphorescence intensity is proportional to the amount of folic acid in the solution, a phosphorimetric method for the determination of folic acid in mice liver was established.
