Effects of nordihydroguaiaretic acid on the expression of GFAP gene of human malignant glioma cell line SHG-44
- VernacularTitle:去甲二氢愈创木酸对SHG-44胶质瘤细胞GFAP基因表达的影响
- Author:
Li YAO
;
Xiuwu BIAN
;
Ziqiang CHEN
- Publication Type:Journal Article
- From:Journal of Third Military Medical University
2001;23(3):254-256
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the expression of glial fibrillary acidic protein (GFAP) gene and its significance in the process of glioma cell differentiation induced by nordihydroguaiaretic acid (NDGA). Methods Immunohistochemistry (IHC) and in situ hybridization were used to detect the expression changes of GFAP protein and GFAP mRNA qualitatively and quantitatively. Results The expression levels of GFAP protein and GFAP mRNA in NDGA treatment group were significantly increased compared with the control group (P<0.01). Conclusion NDGA could induce GFAP gene in malignant glioma cells and the up-regulation of this gene expression might be one of the mechanisms by which NDGA induces glioma differentiation.
