Roles of cyclin-dependent kinase 4 (CDK4) in nordihydroguaiaretic acid-induced inhibitory effect on proliferation of human malignant glioma cells
- VernacularTitle:CDK4在去甲二氢愈创木酸抑制恶性胶质瘤细胞增殖中的作用
- Author:
Deyu GUO
;
Yisheng CHEN
;
Xiuwu BIAN
;
Jingquan SHI
;
Ziqiang CHEN
;
Rong XIN
- Publication Type:Journal Article
- From:Journal of Third Military Medical University
2001;23(3):257-259
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate roles of cyclin-dependent kinase 4 (CDK4) in nordihydroguaiaretic acid (NDGA)-induced inhibitory effect on proliferation of human malignant glioma cells. Methods The techniques of cell culture, cell counts, flow cytometry, immunoprecipitation, immunohistochemistry and image analysis were employed in this study. Results ①A concentration-dependent inhibition of proliferation was demonstrated in the SHG-44 cells incubated for 24 hours in the presence of NDGA, and cell proliferation was blocked in the G1→S phase. ②The activity of CDK4 was decreased apparently in the SHG-44 cells treated for 24 hours with 10 to 200 μmol/L NDGA in a concentration-dependent way. ③The expression of CDK4 gene was downregulated in the cells after NDGA treatment. Conclusion CDK4 plays an important role in NDGA-induced inhibition of glioma cell proliferation.
