Study of genetic variation of the structural region of hepatitis C virus
- VernacularTitle:丙型肝炎病毒结构区基因变异的研究
- Author:
Song CHEN
;
Yuming WANG
- Publication Type:Journal Article
- From:Journal of Third Military Medical University
2001;23(3):296-299
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the role of the structural gene variation of hepatitis C virus(HCV) in the chronicity of HCV infection. Methods Serum samples from four HCV infection acquired during six-month follow up and four patients with chronic hepatitis C type were obtained. A 1-kb fragment containing the 3' half of core ，completed E1 and 5' half of E2/NS1 was amplified by nested reverse transcription polymerase chain reaction (RT-PCR). For each，30 cloned cDNAs were examined by a method that combined heteroduplex analysis (HAD) and a single-stranded conformational polymorphisim (SSCP)assay to optimize selection of clones with unique gel shift patterns (clonotypes )as a measure of the quasispecies complexity. Results There was obvious difference between the SSCP bands of cloned cDNAs representing core-E1 and E2 region，but more distinct difference between heteroduplex and the homoduplex of clones spanning E2 region which accurately reflected the degree of heterogeneity. Quasispecies complexity was higher in those with persistent viremia than those with acute viremia. Conclusion HCV persistence is associated with complex quasispecies in viral structural region.
